推荐产品
ligand
(Sulfoisobutyl)
质量水平
物料
polypropylene housing
产品线
Fractogel®
形式
prepacked column
参数
≤8 bar pressure
~200 cm/hr max. flow rate
column I.D. × L
8 mm × 20 mm
体积
1 mL
基质活性基团
Methacrylate
平均粒径
40-90 μm
容量
~130 mL, resin binding capacity
应用
mAb
recombinant protein
vaccine development
分离技术
cation exchange
储存温度
2-30°C
一般描述
8 x 20mm
特点和优势
MiniChrom Column Fractogel® SO3 (M), 1 ml enables:
Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel® EMD SO3- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).
The strength of the binding depends on the following:
- Increased binding strength at low pH
- High aggregate removal efficiency combined with high dynamic binding capacity
- Lower elution volumes for the highest purity levels
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel® EMD SO3- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).
The strength of the binding depends on the following:
- the buffer system
- pH value of the buffer which determines the surface charge of the protein
- the concentration of the counter ions
- the charge density on the support (protein binding capacity)
分析说明
Appearance: passes test
法律信息
FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany
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