Anti-SIRT2 Antibody

Sirtuins are hypothesized to play a key role in an organism′s response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". Mammals have seven sirtuins; SIRT1 and SIRT2 occupy the cytoplasm. SIRT2 is a NAD-dependent deacytylase. It has been shown to control gene silencing, cell cycle, and DNA damage repair. In additon, SIRT2 acts as a tumor suppressor in human gliomas which suggests that the ectopic expression of SIRT2 upsets the microtubule network.

This antibody recognizes the mature protein of SIRT2.

Epitope: Mature protein

KLH-conjugated linear peptide corresponding to the mature protein of human SIRT2.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

Use Anti-SIRT2 Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect SIRT2 also known as SIR2-like protein 2, silent information regulator 2, sirtuin 2.

Western Blot (SNAP ID) Analysis: 0.25 µg/mL from a representative lot detected SIRT2 on 10 µg of human brain tissue lysate.

Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated SIRT2 in human brain lysate.

Evaluated by Western Blot in human brain tissue lysate.

Western Blot Analysis: 0.125 µg/mL of this antibody detected SIRT2 on 10 µg of human brain tissue lysate.

Observed molecular weight is ~39 kDa Multiple isoforms around 40 kDa may be observed.

Replaces: 04-1124

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Human brain tissue lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.