07-434
抗-BRCA1抗体
serum, Upstate®
别名:
Anti-PNCA4, Anti-PPP1R53, Anti-BRCAI, Anti-BRCC1, Anti-BROVCA1, Anti-FANCS, Anti-IRIS, Anti-PSCP, Anti-RNF53
产品名称
抗-BRCA1抗体, serum, Upstate®
biological source
rabbit
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... BRCA1(672)
Analysis Note
对照
阳性抗原对照：货号12-309，HeLa细胞核提取物。加入等体积的Laemmli样品还原性缓冲液至10 μL提取物中，煮沸5分钟以对样品进行还原。在微凝胶每个泳道上样20 μg经还原的提取物。
阳性抗原对照：货号12-309，HeLa细胞核提取物。加入等体积的Laemmli样品还原性缓冲液至10 μL提取物中，煮沸5分钟以对样品进行还原。在微凝胶每个泳道上样20 μg经还原的提取物。
已通过免疫印迹法对HeLa核提取物进行常规评估
Application
用抗BRCA1抗体（兔多克隆抗体）检测BRCA1，已在IHC、IP & WB中起作用。 这种BRCA1抗体已在人类样本中进行了测试。
研究子类别
细胞周期，DNA 复制&修复
细胞周期，DNA 复制&修复
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
识别人BRCA1。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
220 kDa
已经确定了乳腺癌的两个主要易感基因BRCA1和BRCA2。这两个基因都被认为是肿瘤抑制基因。BRCA1和BRCA2内的突变是大多数家族性乳腺癌病例的原因。BRCA1和BRCA2基因产物的功能分析已经确定了它们在转录调控和DNA损伤修复中的双重参与。 BRCA1通过p53和p21基因影响细胞周期调节，并且由于表达降低而导致的BRCA1功能丧失导致细胞周期停滞。在体内，BRCA1和BRCA2在快速增殖的细胞中以最大水平表达。此特征与BRCA1和BRCA2以细胞周期依赖性方式表达的体外观察结果一致。在乳腺发育过程中，BRCA1和BRCA2的表达在快速增殖的细胞区室中被诱导。
Immunogen
GST标记的融合蛋白对应人BRCA1的1301-1863位氨基酸。
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
含有0.035%叠氮化钠和30％甘油的血清。在-20°C为液体形式。
未纯化
Preparation Note
自收到之日起在-20°C可稳定保存2年。避免反复冻融。为了最大程度地回收产品，在融化后和取下盖子之前，将原始样品瓶进行离心。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
BAL1 and its partner E3 ligase, BBAP, link Poly(ADP-ribose) activation, ubiquitylation, and double-strand DNA repair independent of ATM, MDC1, and RNF8.
Yan, Q; Xu, R; Zhu, L; Cheng, X; Wang, Z; Manis, J; Shipp, MA
Molecular and cellular biology null
Ligand-dependent differences in estrogen receptor beta-interacting proteins identified in lung adenocarcinoma cells corresponds to estrogenic responses.
Ivanova, M; Abner, S; Pierce, W; Klinge, C
Proteome Science null
The chromatin remodeling protein BRG1 modulates BRCA1 response to UV irradiation by regulating ATR/ATM activation.
Zhang, L; Chen, H; Gong, M; Gong, F
Frontiers in Oncology null
Judith J Luciani et al.
Journal of cell science, 119(Pt 12), 2518-2531 (2006-06-01)
We have recently demonstrated that heterochromatin HP1 proteins are aberrantly distributed in lymphocytes of patients with immunodeficiency, centromeric instability and facial dysmorphy (ICF) syndrome. The three HP1 proteins accumulate in one giant body over the 1qh and 16qh juxtacentromeric heterochromatins
Yifan Wang et al.
The Journal of clinical investigation, 126(8), 3145-3157 (2016-07-28)
Patients with cancers that harbor breast cancer 1 (BRCA1) mutations initially respond well to platinum and poly(ADP-ribose) polymerase inhibitor (PARPi) therapy; however, resistance invariably arises in these patients and is a major clinical problem. The BRCA1185delAG allele is a common
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