生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
5.23, monoclonal
种属反应性
human
制造商/商品名称
Upstate®
技术
immunocytochemistry: suitable
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... BRCA2(675)
特异性
BRCA2
免疫原
GST融合蛋白，对应人BRCA2蛋白的2959-3418位残基
应用
用抗BRCA2抗体，克隆5.23（小鼠单克隆抗体）检测BRCA2，已证明可在WB、ICC中起作用。
研究子类别
细胞周期，DNA 复制&修复
细胞周期，DNA 复制&修复
研究类别
表观遗传学&核功能
表观遗传学&核功能
质量
已通过免疫印迹法对HeLa核提取物进行常规评估
目标描述
390kDa
外形
0.1M Tris-甘氨酸（pH 7.4），0.15M NaCl与0.05％叠氮化钠。
形式：纯化
蛋白G层析
储存及稳定性
储存于2-8°C。 收到后，在取下瓶盖之前，将小瓶离心并轻轻混合溶液。分装到微量离心管中，并储存于2-8°C。
分析说明
对照
阳性抗原对照：货号12-309，HeLa细胞核提取物。添加 等体积Laemmli样品还原性缓冲液至10 μL提取物并 煮沸5分钟以还原制剂。在小型凝胶每个泳道上样20 μg 经还原的提取物。
阳性抗原对照：货号12-309，HeLa细胞核提取物。添加 等体积Laemmli样品还原性缓冲液至10 μL提取物并 煮沸5分钟以还原制剂。在小型凝胶每个泳道上样20 μg 经还原的提取物。
其他说明
由于许可协议限制，无法购买该产品进行转售。
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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WGK
WGK 1
Loredana Moro et al.
The Journal of biological chemistry, 281(31), 22100-22107 (2006-06-07)
Aberrant interaction of carcinoma cells with basement membranes (BM) is a fundamental pathophysiological process that initiates a series of events resulting in cancer cell invasion and metastasis. In this report, we describe the results of our investigations pertaining to the
Loredana Moro et al.
The Journal of biological chemistry, 280(23), 22482-22491 (2005-04-05)
BRCA2 is a tumor suppressor gene that when mutated confers an increased susceptibility to developing breast and prostate carcinoma. Besides its role in mediating DNA repair, new evidence suggests that BRCA2 may also play a role in suppressing cancer cell
Jimmy M-M Kwok et al.
Molecular cancer research : MCR, 8(1), 24-34 (2010-01-14)
The transcription factor Forkhead box M1 (FOXM1) is a key regulator of cell proliferation and is overexpressed in many forms of primary cancers, leading to uncontrolled cell division and genomic instability. To address the role of FOXM1 in chemoresistance, we
L Y Marmorstein et al.
Cell, 104(2), 247-257 (2001-02-24)
Germline mutations of the human BRCA2 gene confer susceptibility to breast cancer. Although the function of the BRCA2 protein remains to be determined, murine cells homozygous for BRCA2 inactivation display chromosomal aberrations. We have isolated a 2 MDa BRCA2-containing complex
Miyoung Lee et al.
Nucleic acids research, 49(10), 5588-5604 (2021-05-13)
Cancer-causing missense mutations in the 3418 amino acid BRCA2 breast and ovarian cancer suppressor protein frequently affect a short (∼340 residue) segment in its carboxyl-terminal domain (DBD). Here, we identify a shared molecular mechanism underlying their pathogenicity. Pathogenic BRCA2 missense
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