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生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
CP74, monoclonal
种属反应性
human
制造商/商品名称
Upstate®
技术
immunohistochemistry: suitable
western blot: suitable
同位素/亚型
IgG2bκ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... CDKN1A(1026)
特异性
Cip1
免疫原
Recombinant full-length human Cip1 with an N-terminal histidine tag; epitope corresponds to amino acids 1-80 of full-length human Cip1
应用
Anti-p21/WAF1/Cip1 Antibody, clone CP74 is a high quality Mouse Monoclonal Antibody for the detection of p21/WAF1/Cip1 & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
质量
routinely evaluated by immunoblot on nuclear extract from HeLa cells
目标描述
21kDa
外形
Protein G Purified
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Format: Purified
储存及稳定性
2 years at -20°C
分析说明
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Yongye Huang et al.
Biomedical reports, 7(1), 36-40 (2017-07-08)
Wnt signaling is critical in embryonic development and post-embryonic tissue homeostasis. The aim of the present study was to evaluate the expression levels of canonical Wnt signaling genes in porcine somatic cell nuclear transfer (SCNT) embryos. Quantitative polymerase chain reaction
N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) triggers MSH2 and Cdt2 protein-dependent degradation of the cell cycle and mismatch repair (MMR) inhibitor protein p21Waf1/Cip1.
Jascur, T; Fotedar, R; Greene, S; Hotchkiss, E; Boland, CR
The Journal of Biological Chemistry null
STX140 and STX641 cause apoptosis via the intrinsic mitochondrial pathway and down-regulate survivin and XIAP expression in ovarian and prostate cancer cells.
Foster PA, Ho YT, Newman SP, Leese MP, Potter BV, Reed MJ, Purohit A
Anticancer research null
Z Y Yang et al.
Nature medicine, 1(10), 1052-1056 (1995-10-01)
The p21 gene encodes a cyclin-dependent kinase inhibitor that affects cell-cycle progression, but the potential of this gene product to serve as a tumour suppressor in vivo has not been established. In this report, we show that the growth of
Heat shock protein 90 inhibitors attenuate LPS-induced endothelial hyperpermeability.
Chatterjee, A; Snead, C; Yetik-Anacak, G; Antonova, G; Zeng, J; Catravas, JD
American Journal of Physiology. Lung Cellular and Molecular Physiology null
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