推荐产品
生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
HE12, monoclonal
种属反应性
human
包装
antibody small pack of 25 μg
制造商/商品名称
Upstate®
技术
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG
UniProt登记号
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
human ... CCNE1(898)
一般描述
细胞周期蛋白 E 是 Cdk2 的调节亚基，在哺乳动物细胞周期中控制 G1/S 的转变。细胞周期蛋白 E 的多种同工型仅在肿瘤中表达，而在正常组织中不表达，这表明细胞周期蛋白 E 受转录后调控。体外分析表明，细胞周期蛋白 E 的这些截短的变体能够磷酸化组蛋白 H1。细胞周期蛋白 E 异常被认为是各种癌症预后较差的指标。
特异性
人 p52 周期蛋白 E
免疫原
重组人细胞周期蛋白E
应用
抗细胞周期蛋白 E 抗体，克隆 HE12 是用于检测细胞周期蛋白 E 的高质量小鼠单克隆抗体，&已在 IP、WB、ICC 中进行了验证。
质量
在人 A431 裂解物中的免疫印迹中进行了常规评估
目标描述
52kda
联系
替代：04-223
外形
形式：纯化的
储存及稳定性
在 -20°C 下可保存 2 年，在 4°C 下可保存 6 个月
分析说明
对照
阳性抗原对照：货号12-301，未刺激的A431细胞裂解液。 加入2.5µL 2-巯基乙醇/100µL裂解液，煮沸5分钟以减少制备量。在微凝胶每个泳道上样20µg经还原的裂解液。
阳性抗原对照：货号12-301，未刺激的A431细胞裂解液。 加入2.5µL 2-巯基乙醇/100µL裂解液，煮沸5分钟以减少制备量。在微凝胶每个泳道上样20µg经还原的裂解液。
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
S M Keenan et al.
The Journal of biological chemistry, 276(25), 22404-22409 (2001-04-17)
Activation of cyclin-dependent kinase 2 (CDK2)-cyclin E in the late G(1) phase of the cell cycle is important for transit into S phase. In Chinese hamster embryonic fibroblasts (IIC9) phosphatidylinositol 3-kinase and ERK regulate alpha-thrombin-induced G(1) transit by their effects
Hae-June Lee et al.
Radiation research, 170(5), 579-590 (2008-10-31)
The aim of this work was to identify specific genes involved in rat mammary tumors induced by dimethylbenz(a)anthracene (DMBA) or radiation. More TUNEL- and PCNA-positive cells were present in mammary tumors induced by radiation than in tumors induced by DMBA
Activation of p53 by nutlin leads to rapid differentiation of human embryonic stem cells.
Maimets, T; Neganova, I; Armstrong, L; Lako, M
Oncogene null
Xiaomin Su et al.
BMC developmental biology, 20(1), 22-22 (2020-11-19)
Tissue regeneration mediated by mesenchymal stem cells (MSCs) is deemed a desirable way to repair teeth and craniomaxillofacial tissue defects. Nevertheless, the molecular mechanisms about cell proliferation and committed differentiation of MSCs remain obscure. Previous researches have proved that lysine
Inês Gomes et al.
Oncotarget, 11(19), 1714-1728 (2020-06-02)
The role of RANKL-RANK pathway in progesterone-driven mammary carcinogenesis and triple negative breast cancer tumorigenesis has been well characterized. However, and despite evidences of the existence of RANK-positive hormone receptor (HR)-positive breast tumors, the implication of RANK expression in HR-positive
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