05-166
抗-IGF-II抗体，克隆 S1F2
clone S1F2, Upstate®, from mouse
别名:
Anti-C11orf43, Anti-GRDF, Anti-IGF-II, Anti-PP9974, Anti-SRS3
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
S1F2, monoclonal
Application:
ELISA
immunocytochemistry
immunoprecipitation (IP)
neutralization
western blot
immunocytochemistry
immunoprecipitation (IP)
neutralization
western blot
Species reactivity:
human, rat
Citations:
44
Technique(s):
ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
neutralization: suitable
western blot: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
neutralization: suitable
western blot: suitable
Uniprot accession no.:
产品名称
抗-IGF-II抗体，克隆 S1F2, clone S1F2, Upstate®, from mouse
Quality Level
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
S1F2, monoclonal
species reactivity
human, rat
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
neutralization: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... IGF2(3481)
Analysis Note
通过蛋白质印迹法对 HeLa 酸提取的核制剂（阳性）和重组组蛋白 H3（阴性）进行了评估。
蛋白质印迹分析：
该抗体以 1：5,000：– 1：10,000 的稀释度在 HeLa 核酸提取物中检测到三甲基化组蛋白 H3（Lys4）。
蛋白质印迹分析：
该抗体以 1：5,000：– 1：10,000 的稀释度在 HeLa 核酸提取物中检测到三甲基化组蛋白 H3（Lys4）。
Application
中和：10 μg/mL的先前批次产品抑制了 IGF-II (10 ng/mL) 对鸡胚胎成纤维细胞中DNA合成的刺激作用。 使用 ATP终点分析（ATPLiteÔ-M, Packard Instruments）评估细胞活力。先前批次的该抗体经测定可抑制[3H]-胸苷的IGF-II依赖性结合。
使用经验证可用于ELISA、IP、IC、NEUT& WB的抗-IGF-II抗体（克隆S1F2）检测IGF-II。
研究子类别
胰岛素/能量信号
胰岛素/能量信号
研究类别
信号传导
信号传导
Biochem/physiol Actions
与人IGF-I的交叉反应性低于10%(Tanaka, H, 1989; Suzuki, T, 1989)。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
~7 kDa
人胰岛素样生长因子II （IGFII）含有67个氨基酸，结构特性类似于IGFI，包括62%的序列同源性。 在人血浆中，IGFI和 IGFII 与IGF结合蛋白相结合，IGF结合蛋白可运输多肽并部分调节其体内作用。除了胰岛素受体，IGFII 结合两种形式的 IGF受体，两种受体都在不同组织和培养的细胞中广泛分布。对于各种培养的细胞，包括小鼠3T3 细胞、12种正常大鼠肾细胞、人或鸡成纤维细胞以及MCF-7人乳腺癌细胞，IGFII 有促有丝分裂作用。
Immunogen
部分纯化的大鼠 IGF-II。克隆S1F2
Other Notes
浓度：请参考批次特异性浓缩物的检验报告。
Physical form
形式：纯化
纯化的小鼠单克隆IgG1 ，溶于含PBS（pH 7.4）的缓冲液中，通过硫酸铵沉淀和DEAE-纤维素色谱法纯化。 冷冻溶液。
Preparation Note
自收到之日起在-20ºC可稳定保存1年。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Tanaka, H, et al
Endocrinology, 124, 870-877 (1989)
Combined magnetic fields increase insulin-like growth factor-II in TE-85 human osteosarcoma bone cell cultures.
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