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Merck
CN

04-642

抗Ago2抗体，克隆9E8.2

ascites fluid, clone 9E8.2, Upstate®

别名:

Argonaute-2, Eukaryotic translation initiation factor 2C, AGO2, eIF-2C, Slicer protein, MGC3183, Piwi domain protein, PPD

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关于此项目

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

主要文件

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生物来源

mouse

质量水平

100

偶联物

unconjugated

抗体形式

ascites fluid

抗体产品类型

primary antibodies

克隆

9E8.2, monoclonal

种属反应性

human

制造商/商品名称

Upstate®

技术

ChIP: suitable (ChIP-seq)
western blot: suitable

同位素/亚型

IgG1κ

NCBI登记号

NM_012154

UniProt登记号

Q9UKV8

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... AGO2(27161)

相关类别

一般描述

Ago2（argonaute-2），也称为真核翻译起始因子2C（EIF2C2），是siRNA定向RNA干扰（RNAi）反应的重要组成部分。 Ago2是解开siRNA双链体和将siRNA组装成RNA诱导的沉默复合物（RISC）所需的核酸内切酶。 Ago2通过其Piwi域与DICER1相互作用。 该Piwi结构域被认为通过类似于RNase H的机制提供RNA切割活性。Ago2活性对于胚胎发育以及RNA介导的基因沉默（RNAi）是必需的。
~100 kda

免疫原

KLH偶联的合成肽，包含序列YSGAGPALAPPAPPPPIQG
表位：在Ago2的N端附近

应用

ChIP和ChIP-seq：该抗体的代表性批次用于执行ChIP和ChIP-seq（Woolnough, JL, Atwood, BL, and Giles KE (2015), MCB Vol. 35 No. 13, p. 2278-2294）。
抗Ago2抗体，克隆9E8.2是一种小鼠单克隆抗体，用于检测Ago2（也称为Argonaute-2，真核翻译起始因子2C），&已在WB中验证，并已证明可在ChIP和ChIP-seq中执行。
研究子类别
RNA代谢&结合蛋白

染色质生物

RNA结合蛋白（RBP）
研究类别
表观遗传学&核功能

生化/生理作用

人Ago2

外形

免疫亲和纯化
含0.05％叠氮化钠的腹水

制备说明

自收到之日起，在-20°C条件下可稳定保存1年。
处理建议：收到后，在取下瓶盖之前，将小瓶离心并轻轻混合溶液。分装到微量离心管中，并储存于 -20°C。避免反复冻融循环，以免损坏IgG和影响产品性能

分析说明

已通过免疫印迹进行常规评估。

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

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Li Zhang et al.
Cell death & disease, 10(3), 168-168 (2019-02-20)
Cholestasis induces the hepatic long non-coding RNA H19, which promotes the progression of cholestatic liver fibrosis. However, microRNAs that are dysregulated by H19 during cholestasis remain elusive. Using miRNA-sequencing analysis followed by qPCR validation, we identified marked upregulation of eight
Keriayn N Smith et al.
Stem cell reports, 9(1), 108-121 (2017-06-06)
Of the thousands of long noncoding RNAs expressed in embryonic stem cells (ESCs), few have known roles and fewer have been functionally implicated in the regulation of self-renewal and pluripotency, or the reprogramming of somatic cells to the pluripotent state.
Simon J Allison et al.
Molecular therapy. Nucleic acids, 2, e141-e141 (2014-01-09)
Selective gene silencing by RNA interference (RNAi) involves double-stranded small interfering RNA (ds siRNA) composed of single-stranded (ss) guide and passenger RNAs. siRNA is recognized and processed by Ago2 and C3PO, endonucleases of the RNA-induced silencing complex (RISC). RISC cleaves
Differential expression of microRNA expression in tamoxifen-sensitive MCF-7 versus tamoxifen-resistant LY2 human breast cancer cells.
Manavalan, TT; Teng, Y; Appana, SN; Datta, S; Kalbfleisch, TS; Li, Y; Klinge, CM
Cancer letters null
Hanane Ennajdaoui et al.
Cell reports, 15(9), 1876-1883 (2016-05-24)
Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) expression correlates with malignancy, but its role(s) in pathogenesis remains enigmatic. We interrogated the IGF2BP3-RNA interaction network in pancreatic ductal adenocarcinoma (PDAC) cells. Using a combination of genome-wide approaches, we have
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