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04-642

抗Ago2抗体，克隆9E8.2

ascites fluid, clone 9E8.2, Upstate®

别名:

Argonaute-2, Eukaryotic translation initiation factor 2C, AGO2, eIF-2C, Slicer protein, MGC3183, Piwi domain protein, PPD

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
9E8.2, monoclonal
Application:
ChIP, WB
Citations:
23
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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

9E8.2, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq), western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_012154

UniProt accession no.

Q9UKV8

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... AGO2(27161)

General description

Ago2（argonaute-2），也称为真核翻译起始因子2C（EIF2C2），是siRNA定向RNA干扰（RNAi）反应的重要组成部分。 Ago2是解开siRNA双链体和将siRNA组装成RNA诱导的沉默复合物（RISC）所需的核酸内切酶。 Ago2通过其Piwi域与DICER1相互作用。 该Piwi结构域被认为通过类似于RNase H的机制提供RNA切割活性。Ago2活性对于胚胎发育以及RNA介导的基因沉默（RNAi）是必需的。
~100 kda

Immunogen

KLH偶联的合成肽，包含序列YSGAGPALAPPAPPPPIQG
表位：在Ago2的N端附近

Application

ChIP和ChIP-seq：该抗体的代表性批次用于执行ChIP和ChIP-seq（Woolnough, JL, Atwood, BL, and Giles KE (2015), MCB Vol. 35 No. 13, p. 2278-2294）。
抗Ago2抗体，克隆9E8.2是一种小鼠单克隆抗体，用于检测Ago2（也称为Argonaute-2，真核翻译起始因子2C），&已在WB中验证，并已证明可在ChIP和ChIP-seq中执行。
研究子类别
RNA代谢&结合蛋白

染色质生物

RNA结合蛋白（RBP）
研究类别
表观遗传学&核功能

Biochem/physiol Actions

人Ago2

Physical form

免疫亲和纯化
含0.05％叠氮化钠的腹水

Preparation Note

自收到之日起，在-20°C条件下可稳定保存1年。
处理建议：收到后，在取下瓶盖之前，将小瓶离心并轻轻混合溶液。分装到微量离心管中，并储存于 -20°C。避免反复冻融循环，以免损坏IgG和影响产品性能

Analysis Note

已通过免疫印迹进行常规评估。

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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