03-0202-00
SMC® Human NF-L High Sensitivity Immunoassay Kit
Used for ultrasensitive quantification of Human NF-L in human serum, plasma, and cerebrospinal fluid (CSF)
别名:
NF-L Single Molecule Counting kit, NF-L immunoassay kit, NF-L ultra-sensitive immunoassay, Neurofilament Light Chain kit, NfL kit
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NACRES:
NA.77
UNSPSC Code:
12161503
产品名称
SMC® Human NF-L High Sensitivity Immunoassay Kit,
Quality Segment
description
Used for ultrasensitive quantification of Human NF-L in human serum, plasma, and cerebrospinal fluid (CSF)
product line
SMC®
usage
kit sufficient for 96 wells
species reactivity
human, human
parameter
25 μL sample volume (CSF), 50 μL sample volume (serum and plasma)
technique(s)
single molecule counting: suitable
input
plasma (K2 EDTA)
cerebrospinal fluid (CSF)
serum
plasma (K2 EDTA)
cerebrospinal fluid (CSF)
serum
UniProt accession no.
compatibility
for use with FemtoQuest™ and SMCxPRO® instruments
shipped in
wet ice
storage temp.
2-8°C
General description
Neurofilament light chain (NF-L) is a critical component of the neurofilament cytoskeleton in neurons, providing structural support and maintaining neuronal integrity. As part of the larger family of neurofilament proteins, NF-L, along with neurofilament medium chain (NF-M) and neurofilament heavy chain (NF-H), contributes to the formation of intermediate filaments that are essential for the structural stability of axons. NF-L plays an essential role in axonal transport, facilitating the movement of organelles and proteins along the axon, which is vital for neuronal function and communication.
Additionally, NF-L participates in the response to injury and stress, helping neurons adapt to changing conditions. Its presence in the central nervous system is crucial for maintaining the overall health and functionality of neurons. NF-L serves as a valuable biomarker for neuronal damage and neuroinflammation. Elevated levels of NF-L in cerebrospinal fluid (CSF) and blood indicate ongoing neurodegeneration and correlate with conditions such as Alzheimer′s disease, amyotrophic lateral sclerosis (ALS), and multiple sclerosis (MS). Additionally, increased NF-L levels often reflect the severity of neuroinflammatory processes. The measurement of NF-L is essential for developing therapeutic strategies aimed at preserving neuronal health and function in various neurological disorders.
Additionally, NF-L participates in the response to injury and stress, helping neurons adapt to changing conditions. Its presence in the central nervous system is crucial for maintaining the overall health and functionality of neurons. NF-L serves as a valuable biomarker for neuronal damage and neuroinflammation. Elevated levels of NF-L in cerebrospinal fluid (CSF) and blood indicate ongoing neurodegeneration and correlate with conditions such as Alzheimer′s disease, amyotrophic lateral sclerosis (ALS), and multiple sclerosis (MS). Additionally, increased NF-L levels often reflect the severity of neuroinflammatory processes. The measurement of NF-L is essential for developing therapeutic strategies aimed at preserving neuronal health and function in various neurological disorders.
Application
Alzheimer′s Disease / Aging / Nervous System / Neurodegenerative Diseases / Neurofilament & Neuron Metabolism / Neuronal & Glial Markers / Neurodegenerative Medicine / Neuroscience
Features and Benefits
- High Sensitivity Detection: Precisely quantifies NF-L at femtogram/mL levels in serum, plasma or CSF.
- Improved Dynamic Range: Enhanced signal-to-noise ratio, reducing background interference for reliable assay performance.
- User-Friendly Workflow: Utilizes a familiar sandwich immunoassay format while ensuring high sensitivity detection.
- Automation-Ready: Compatible with automated liquid handling systems, reducing manual steps and increasing lab efficiency.
- Dedicated Technical Support: Backed by experts experienced in SMC® assay technology.
Packaging
96 well assay
Analysis Note
- Sensitivity: LLOQ 870 fg/mL* (SMCxPRO® Instrument)
- Standard Curve Range: 430-1,500,000 fg/mL
- Intra-assay CV: <15%
- Inter-assay CV: <20%
- Linearity: Serum: 110% Plasma: 106% CSF: 101%
- Recovery: Serum: 93% Plasma: 94% CSF: 123%
Other Notes
Although NF-L was detectable in mouse, non-human primate (rhesus macaque and cynomolgus monkey), and canine samples, the assay has not been verified for these species.
Legal Information
FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
signalword
Danger
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B