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比较 (4)
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without L-glutamine, liquid, sterile-filtered, suitable for cell culture
liquid, sterile-filtered, suitable for cell culture
HEPES Modification, With 25 mM HEPES and stable glutamine, liquid, sterile-filtered, suitable for cell culture
HEPES Modification, With 25 mM HEPES and stable glutamine, liquid, sterile-filtered
说明
属性
components:
L-glutamine: nocomponents:
L-glutamine: noglucose: 4500 mg/L (Dextro)
sodium pyruvate: yes
components:
stable glutamine: yesHEPES: 25 mM
phenol red: yes
NaHCO3: yes
components:
stable glutamine: yesphenol red: yes
HEPES: 25 mM
NaHCO3: yes
shipped in:
ambientshipped in:
ambientshipped in:
ambientshipped in:
ambientimpurities:
endotoxin, testedimpurities:
endotoxin, testedimpurities:
impurities:
form:
liquidform:
liquidform:
liquidform:
liquidsterility:
sterile-filteredsterility:
sterile-filteredsterility:
sterile-filteredsterile
sterility:
sterile-filteredsterile
storage temp.:
2-8°Cstorage temp.:
2-8°Cstorage temp.:
2-8°Cstorage temp.:
2-8°Ctechnique(s):
cell culture | mammalian: suitabletechnique(s):
cell culture | mammalian: suitabletechnique(s):
cell culture | mammalian: suitabletechnique(s):
cell culture | mammalian: suitableQuality Level:
500Quality Level:
300Quality Level:
400Quality Level:
400用途与安全性
应用
MegaCell™ RPMI-1640 Medium has been used:
- as a component of the TSC medium for culturing trophoblast stem cells (TSC)
- as a component of 25 ng/ml FGF4 and 1 g/ml heparin (TSF4H medium) for culturing TSCs
- as a component of embryo-like structures (ETS) embryo medium and TS cell medium for culturing embryonic stem cells (ESCs) and TSCs
MegaCell™ Dulbecco′s Modified Eagle′s Medium has been used:
- as a component of the M10 medium to culture CD133+ cells from biopsies of para-spinal and quadriceps muscle of Duchenne muscular dystrophy patients
- as a component of the proliferation medium for the expansion and cryopreservation of human induced pluripotent stem (iPS) cells cultured without feeder cells and human embryonic (ES) cell-derived mesoangioblast-like cells
- to culture the positive hybridomas during the production of mouse anti-chicken CD25 monoclonal antibodies
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.
风险与安全
Storage Class Code:
10 - Combustible liquids
WGK:
WGK 3
Flash Point(F):
Not applicable
Flash Point(C):
Not applicable
Storage Class Code:
12 - Non Combustible Liquids
WGK:
WGK 1
Flash Point(F):
Not applicable
Flash Point(C):
Not applicable
Storage Class Code:
12 - Non Combustible Liquids
WGK:
WGK 1
Flash Point(F):
Not applicable
Flash Point(C):
Not applicable
Storage Class Code:
12 - Non Combustible Liquids
WGK:
WGK 1
Flash Point(F):
Not applicable
Flash Point(C):
Not applicable
