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powder, BioReagent, suitable for cell culture, suitable for insect cell culture
solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture
certified reference material, pharmaceutical secondary standard
puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.7%
说明
别名
Sodium hydrogen carbonate, soda bicarbonate
aqueous sodium bicarbonate
Monosodium hydrogen carbonate, Sodium hydrogen carbonate
Sodium hydrogen carbonate
CAS号
分子式
线性分子式
: NaHCO3线性分子式
: NaHCO3线性分子式
: NaHCO3经验公式（希尔记法）
: CHNaO3经验公式（希尔记法）
: CHNaO3经验公式（希尔记法）
: CHNaO3分子量
: 84.01分子量
: 84.01分子量
: 84.01MDL号
MFCD00003528
MFCD00003528
MFCD00003528
MFCD00003528
质量目标
属性
Agency:
Agency:
Agency:
traceable to USP 1613655Agency:
reag. Ph. Eur.anion traces:
anion traces:
anion traces:
anion traces:
chloride (Cl-): ≤0.003%phosphate (PO43-): ≤10 mg/kg
Assay:
99.5-100.5%Assay:
Assay:
Assay:
≥99.7%cation traces:
cation traces:
cation traces:
cation traces:
As: ≤0.0002%Ca: ≤0.01%
Cd: ≤0.0005%
Co: ≤0.005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.005%
Ni: ≤0.005%
Pb: ≤0.0005%
Zn: ≤0.0005%
heavy metals: ≤5 mg/kg (by ICP-OES)
density:
2.16 g/mL at 25 °C (lit.)density:
density:
2.16 g/mL at 25 °C (lit.)density:
2.16 g/mL at 25 °C (lit.)impurities:
impurities:
endotoxin, testedimpurities:
impurities:
≤0.003% S-compounds (as SO4)≤0.015% in water insoluble matters
≤5 mg/kg ammonium (NH4)
≤50 mg/kg I red. matters (as SO2)
InChI:
1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1InChI:
1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1InChI:
1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1InChI:
1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1InChI key:
UIIMBOGNXHQVGW-UHFFFAOYSA-MInChI key:
UIIMBOGNXHQVGW-UHFFFAOYSA-MInChI key:
UIIMBOGNXHQVGW-UHFFFAOYSA-MInChI key:
UIIMBOGNXHQVGW-UHFFFAOYSA-Mapplication(s):
application(s):
application(s):
pharmaceuticalapplication(s):
packaging:
packaging:
packaging:
pkg of 3Gpackaging:
pH:
6.8-7.4pH:
7.8-8.2pH:
pH:
≤8.6 (20 °C, 5%)form:
powderform:
solution (7.5%)form:
form:
powderpKa (25 °C):
(1) 6.37, (2) 10.25 (carbonic acid)pKa (25 °C):
pKa (25 °C):
(1) 6.37, (2) 10.25 (carbonic acid)pKa (25 °C):
(1) 6.37, (2) 10.25 (carbonic acid)product line:
BioReagentproduct line:
BioReagentproduct line:
product line:
grade:
Molecular Biologygrade:
grade:
certified reference materialpharmaceutical secondary standard
grade:
ACS reagentpuriss. p.a.
quality:
300quality:
quality:
quality:
SMILES string:
[Na+].OC([O-])=OSMILES string:
[Na+].OC([O-])=OSMILES string:
[Na+].OC([O-])=OSMILES string:
[Na+].OC([O-])=Osolubility:
water: soluble 100 mg/mLsolubility:
solubility:
solubility:
H2O: 0.5 g/10 mL, clear, colorlesswater: soluble 50 g/L
sterility:
sterility:
sterile-filteredsterility:
sterility:
storage temp.:
storage temp.:
2-8°Cstorage temp.:
2-30°Cstorage temp.:
suitability:
suitability:
suitability:
suitability:
complies for appearance of solutiontechnique(s):
cell culture | insect: suitablecell culture | mammalian: suitable
technique(s):
cell culture | mammalian: suitabletechnique(s):
technique(s):
Quality Level:
300Quality Level:
400Quality Level:
300Quality Level:
用途与安全性
应用
Sodium bicarbonate has been used:
- as a component of bovine embryo culture system during in vitro maturation, fertilization, and culture (IVMFC)
- as a component of a 1:1 mixture of Dulbecco′s modified Eagle′s medium and Ham′s nutrient mixture F-12 during the culture of bovine microvascular endothelial cells
- as a component of ITS (insulin transferrin and selenite) medium used during the isolation of dopamine neurons from human embryonic stem cells
- as a component of medium used for cell culture of human embryonic kidney cells
- as a component of Beltsville thawing solution used for the dilution of semen
Sodium bicarbonate solution has been used:
- as a component of Hanks′ buffered salt solution (HBSS) and recording medium for culturing of tissue explants from rats or mice
- as a component of Dulbecco′s modified Eagle′s medium (DMEM) to culture mice tissue explants
- as a component of Minimum Essential Media (MEM) to study the cytotoxicity against human colorectal adenocarcinoma cells and mammary gland adenocarcinoma
- to prepare collagen mix droplets for 3D collagen sprouting assay
- to prepare collagen matrix for rat epidermal keratinocyte (REK) organotypic culture
These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements.
Sodium bicarbonate may be used to maintain 7.4pH in Iscove′s modified Dulbecco′s medium for growth of murine hybridoma cell culture. It may be employed as a component of RPMI-1640 liquid medium in a study.
风险与安全
Storage Class Code:
11 - Combustible Solids
WGK:
WGK 1
Flash Point(F):
Not applicable
Flash Point(C):
Not applicable
Personal Protective Equipment:
Eyeshields, Gloves, type N95 (US)
Storage Class Code:
12 - Non Combustible Liquids
WGK:
WGK 1
Flash Point(F):
Not applicable
Flash Point(C):
Not applicable
Storage Class Code:
13 - Non Combustible Solids
WGK:
WGK 1
Flash Point(F):
Not applicable
Flash Point(C):
Not applicable
Storage Class Code:
13 - Non Combustible Solids
WGK:
WGK 1
Flash Point(F):
Not applicable
Flash Point(C):
Not applicable
