推荐产品
一般描述
蛋白质组学动态范围标准品套装由48种单独的人源或人序列重组蛋白的混合物产生，每种重组蛋白都被选择用于限制异源翻译后修饰（PTM）。蛋白质标准品由6种蛋白质的6种混合物配制而成，其动态范围为5个数量级，范围为50皮摩尔至500摩尔。在配制之前，通过氨基酸分析（AAA）对每种蛋白质进行定量。
应用
蛋白组学动态范围标准套件用于基于强度的绝对定量（iBAQ），适用 大肠杆菌 蛋白、 胚胎干细胞（ESC）和神经元前体细胞（NPC）蛋白组。 也作为标准品，无标记定量刺突 HeLa 细胞。
蛋白质组学动态范围标准品套装可用于在分析复杂蛋白质样品之前标准化和/或评估质谱（例如，LC-MS / MS，MALDI-TOF-MS等）和电泳分析条件。UPS2 可用于在已知复杂标准样品的运行之间交叉包含宝贵的实验数据集。从而确认分析方法的耐用性和所用仪器的稳定性。此外，生成或比较来自定义不明确的样品的质谱数据的实验室可以使用UPS2作为外部参考，以协助评估结果和实验方法。
蛋白质组学动态范围标准品套装已用于在 Orbitrap 分析仪上使用全离子碎片对动态范围通用蛋白质标准品进行定量。它已被用作LC-MS（液相色谱 - 质谱）/ MS分析中基于强度的蛋白质绝对定量（iBAQ）的标准。
蛋白质组学动态范围标准品套装已用于在 Orbitrap 分析仪上使用全离子碎片对动态范围通用蛋白质标准品进行定量。它已被用作LC-MS（液相色谱 - 质谱）/ MS分析中基于强度的蛋白质绝对定量（iBAQ）的标准。
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μg化学品安全说明书
警示用语：
Danger
危险分类
Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
高风险级别生物产品--人源产品
Boumediene Soufi et al.
Frontiers in microbiology, 6, 103-103 (2015-03-06)
We set out to provide a resource to the microbiology community especially with respect to systems biology based endeavors. To this end, we generated a comprehensive dataset monitoring the changes in protein expression, copy number, and post translational modifications in
Jürgen Cox et al.
Molecular & cellular proteomics : MCP, 13(9), 2513-2526 (2014-06-20)
Protein quantification without isotopic labels has been a long-standing interest in the proteomics field. However, accurate and robust proteome-wide quantification with label-free approaches remains a challenge. We developed a new intensity determination and normalization procedure called MaxLFQ that is fully
Taejoon Kwon et al.
Journal of proteome research, 10(7), 2949-2958 (2011-04-15)
Shotgun proteomics using mass spectrometry is a powerful method for protein identification but suffers limited sensitivity in complex samples. Integrating peptide identifications from multiple database search engines is a promising strategy to increase the number of peptide identifications and reduce
Mari J Aaltonen et al.
The Journal of cell biology, 213(5), 525-534 (2016-06-01)
Mitochondria exert critical functions in cellular lipid metabolism and promote the synthesis of major constituents of cellular membranes, such as phosphatidylethanolamine (PE) and phosphatidylcholine. Here, we demonstrate that the phosphatidylserine decarboxylase Psd1, located in the inner mitochondrial membrane, promotes mitochondrial
Björn Schwanhäusser et al.
Nature, 473(7347), 337-342 (2011-05-20)
Gene expression is a multistep process that involves the transcription, translation and turnover of messenger RNAs and proteins. Although it is one of the most fundamental processes of life, the entire cascade has never been quantified on a genome-wide scale.
商品
High-throughput proteomics advances with improved analysis methods and mass spectrometry.
相关内容
Standardize research with Universal and Dynamic Proteomics Standards, complex and well-characterized reference standards for mass spectrometry.
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