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SEQR

Sigma-Aldrich

SeqPlex RNA扩增试剂盒

For use with high throughput sequencing technologies

别名:

WGA 试剂盒

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About This Item

UNSPSC代码:
41121800
NACRES:
NA.55
价格与库存信息目前不能提供

质量水平

200

技术

whole genome amplification: suitable

运输

wet ice

储存温度

−20°C

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form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

foreign activity

β-Glucosidase, α−galactosidase and β−galactosidase ≤0.1%

foreign activity

-

foreign activity

β-Glucosidase, α−galactosidase and β−galactosidase ≤0.1%

foreign activity

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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greener alternative product characteristics

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greener alternative product characteristics

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greener alternative product characteristics

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Learn more about the Principles of Green Chemistry.

一般描述

用于全转录组扩增(WTA)的SeqPlex™RNA扩增试剂盒(SEQXE)专用于对少量或降解/高度片段化的RNA（如来自福尔马林固定石蜡包埋（FFPE）组织样本的RNA ）进行下一代测序(NGS)。通过SeqPlex试剂盒可对这类RNA以及其它用于NGS的少量/高度片段化RDNA样品进行预扩增。它还有助于扩增从组织、培养细胞、福尔马林固定样品或血清中分离的非 polyA 尾 RNA，同时保持未扩增样品中发现的差异表达模式。

应用

SeqPlex™ RNA扩增试剂盒已用于扩增poly（A）筛选的RNA。[1][2]

特点和优势

  • 少量总 RNA 随机引物技术可扩增来自所有来源（包括 FFPE 和 RIP）的片段化或完整 RNA。
  • 半简并文库引物设计，可实现更完整的转录组覆盖和高效引发。
  • 测序前无需进行 DNA的片段化。
  • 在 8 小时或更短的时间内扩增 ds-cDNA。
  • 兼容Pacific Bioscience以外的其它所有下一代测序平台。

其他说明

SEQR-500RXN 按需生产。请联系我们获取更多信息。

法律信息

SeqPlex is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

11 - Combustible Solids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000401679
0000401679
SLCQ9363
SLCQ1302
SLCQ9365

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Kathryn D Tuttle et al.
Nature communications, 9(1), 2650-2650 (2018-07-10)
During development in the thymus, invariant natural killer T (iNKT) cells commit to one of three major functionally different subsets, iNKT1, iNKT2, and iNKT17. Here, we show that T cell antigen receptor (TCR) signal strength governs the development of iNKT
Grégory Seumois et al.
Nature immunology, 15(8), 777-788 (2014-07-07)
A characteristic feature of asthma is the aberrant accumulation, differentiation or function of memory CD4(+) T cells that produce type 2 cytokines (TH2 cells). By mapping genome-wide histone modification profiles for subsets of T cells isolated from peripheral blood of
Isaac Engel et al.
Nature immunology, 17(6), 728-739 (2016-04-19)
Natural killer T cells (NKT cells) have stimulatory or inhibitory effects on the immune response that can be attributed in part to the existence of functional subsets of NKT cells. These subsets have been characterized only on the basis of
DNA Sequencing Research Group (DSRG): Evaluation of RNA Amplification Kits at Subnanogram Input Amounts of Total RNA for RNA-Seq
Nicolet C, et al.
Journal of biomolecular techniques : JBT, 24, S70-S70 (2013)
Giuliana P Mognol et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(13), E2776-E2785 (2017-03-12)
T-cell exhaustion is a progressive loss of effector function and memory potential due to persistent antigen exposure, which occurs in chronic viral infections and cancer. Here we investigate the relation between gene expression and chromatin accessibility in CD8+ tumor-infiltrating lymphocytes
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实验方案

SEQR - SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.

SEQR – SeqPlex RNA扩增试剂盒实验方案

SeqPlex RNA扩增试剂盒可在进入常用深度测序平台工作流程之前为总RNA或分离后的mRNA提供一种扩增方法，

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