产品线
BioUltra
质量水平
形式
(40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)
比活
≥5,700 DEA units/mg protein
分子量
dimer ~160 kDa
浓度
≥10 mg/mL
运输
wet ice
储存温度
2-8°C
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相关类别
一般描述
牛肠粘膜碱性磷酸酶既是磷酸单酯酶，又是焦磷酸酶。
应用
推荐用于抗体和蛋白质缀合的高比活性等级。
牛肠粘膜碱性磷酸酶已被用于测量具有不同氟烃链长度的三种多氟代烷基磷酸单酯的水解动力学。 另一项研究表明，其异构体C-1和C-2的发育调控表达预示了肠道碱性磷酸酶随组织成熟而发生的功能变化。
碱性磷酸酶可用于酪蛋白和其他蛋白质的去磷酸化。碱性磷酸酶还可用于 DNA 或 RNA 的 5′-末端的去磷酸化，以防止自我连接。经碱性磷酸酶的去磷酸化作用后，DNA 或 RNA 还可被放射性标记磷酸盐标记（通过 T4 多核苷酸激酶）。
生化/生理作用
碱性磷酸酶具有抗炎作用，通过作用于脂质A发挥解毒作用而降低感染性休克的严重程度。
该酶对醇类、胺类、焦磷酸盐以及酚类的磷酸酯具有广泛的特异性。常用于蛋白质和核酸的去磷酸化。
物理属性
牛肠碱性磷酸酶为二聚膜衍生糖蛋白。至少存在三种同工酶，每个单体通常拥有两个 N-连接多糖和一个或多个 O-连接多糖。2 该酶需要锌以及镁或钙二价离子才能显示活性。
牛肠道碱性磷酸酶是一种二聚化的膜衍生糖蛋白。其存在至少三种异构体，每个单体通常具有两个N-连接的和一个或多个O-连接的聚糖。
单位定义
在pH 9.8,37℃条件下，一个DEA单位可每分钟水解1 μmole的4-硝基苯基磷酸酯。（一个甘氨酸单位相当于~3个DEA单位）
外形
40% 甘油溶液，含 6 mM Tris、6 mM MgCl2 和 0.12 mM ZnCl2，pH值为 7.6
分析说明
包装规格取决于 DEA 单位
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
动植物源性产品
Suzanne Kats et al.
Recent patents on inflammation & allergy drug discovery, 3(3), 214-220 (2009-06-19)
Laboratory and clinical data have implicated endotoxin as an important factor in the inflammatory response to cardiopulmonary bypass. Alkaline phosphatase prevents endotoxin-induced systemic inflammation in animals and humans. We assessed the effects of the administration of bovine intestinal alkaline phosphatase
M Besman et al.
The Journal of biological chemistry, 260(20), 11190-11193 (1985-09-15)
Alkaline phosphatases from calf and bovine small intestines have been isolated in homogeneous form from both mucosa and luminal contents. The detergent-solubilized calf enzyme resolves into two peaks of activity, C-1 and C-2, on chromatofocusing. Only one of these activity
Dagmara Wojcik-Grzybek et al.
International journal of molecular sciences, 23(6) (2022-03-26)
Inflammatory bowel diseases (IBD) are commonly considered as Crohn's disease and ulcerative colitis, but the possibility that the alterations in gut microbiota and oxidative stress may affect the course of experimental colitis in obese physically exercising mice treated with the
Fuhong Su et al.
Critical care medicine, 34(8), 2182-2187 (2006-06-16)
Alkaline phosphatase may decrease the harmful effects of lipopolysaccharide by detoxifying lipid A. The aim of this study was to investigate whether administration of alkaline phosphatase is beneficial in a clinically relevant septic shock model. Interventional laboratory study. University hospital
Senem Aykul et al.
International journal of molecular sciences, 22(16) (2021-08-28)
Adipose tissues (AT) expand in response to energy surplus through adipocyte hypertrophy and hyperplasia. The latter, also known as adipogenesis, is a process by which multipotent precursors differentiate to form mature adipocytes. This process is directed by developmental cues that
实验方案
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
碱性磷酸酶的酶法测定，二乙醇胺法（EC 3.1.3.1)
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