CN¥4,638.25
预计发货时间May 16, 2025详情
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标签
Met-FLAG tagged
等级
Molecular Biology
表单
buffered aqueous solution
肽标签位置
N-terminal
运输
dry ice
储存温度
−20°C
一般描述
pFLAG-CMV™-4是一个6.2 kb的表达载体，用于哺乳动物细胞中的瞬时或稳定表达。该载体是一种pCMV5的衍生物，用于将正确插入的开放阅读框作为N末端Met-FLAG®融合蛋白进行瞬时或稳定表达。
pFLAG-CMV-4表达载体是一种穿梭载体，包含细菌和SV40复制起点，可在大肠杆菌和哺乳动物细胞中增殖。当使用表达SV40大T抗原的宿主细胞时，复制和基因组整合的效率最高。
FLAG表位是一个由8个氨基酸标签（DYKDDDDK）组成的小型亲水性标记物，可使用于Sigma Aldrich的ANTI-FLAG产品进行灵敏检测和高质量纯化。
pFLAG-CMV-4-BAP对照质粒是pCMV5的7.7 kb衍生物，用于N末端Met-FLAG细菌碱性磷酸酶融合蛋白在哺乳动物细胞中的瞬时细胞内表达。
载体图谱和序列
pFLAG-CMV-4表达载体是一种穿梭载体，包含细菌和SV40复制起点，可在大肠杆菌和哺乳动物细胞中增殖。当使用表达SV40大T抗原的宿主细胞时，复制和基因组整合的效率最高。
FLAG表位是一个由8个氨基酸标签（DYKDDDDK）组成的小型亲水性标记物，可使用于Sigma Aldrich的ANTI-FLAG产品进行灵敏检测和高质量纯化。
pFLAG-CMV-4-BAP对照质粒是pCMV5的7.7 kb衍生物，用于N末端Met-FLAG细菌碱性磷酸酶融合蛋白在哺乳动物细胞中的瞬时细胞内表达。
载体图谱和序列
组分
- pFLAG-CMV™-4 表达载体 20 μg (E1775) ，浓度为 0.5mg/ml，溶于含有 1mM EDTA 的 10mM Tris-HCl（pH8.0）中。
- pFLAG-CMV™-4-BAP 对照质粒 20 μg (C4722) ，浓度为 0.5mg/ml，溶于含有 1mM EDTA 的 10mM Tris-HCl（pH8.0）中。
原理
人巨细胞病毒2的启动子调节区可驱动FLAG®-融合构建体的转录。氨基糖苷磷酸转移酶II基因（neo-r）可表现对氨基糖苷类（如G 418）的抗性，从而可用于稳定转染子的选择。
法律信息
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
储存分类代码
10 - Combustible liquids
法规信息
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