推荐产品
等级
for molecular biology
质量水平
形式
powder blend
技术
Southern blotting: suitable
pH值（酸碱度）
6.8-7.2(reconstituted per directions)
溶解性
H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)
储存温度
room temp
一般描述
酪蛋白是一种牛奶来源的物质，通常用于 Western、Southern 和 Northern 印迹实验中的封闭缓冲液。封闭剂用于减少检测试剂与膜的非特异性结合。
应用
适合用作 Western、Southern 和 Northern 印迹膜的封闭缓冲液。然后，封闭膜可用于化学发光，比色或 DIG 检测分析。
特点和优势
- 封闭性能优于奶粉或 BSA
- 与中性和正电荷膜兼容
- 与化学发光，比色和 DIG 检测分析兼容
制备说明
将瓶中的全部内容物溶解在 800 mL 分子生物学级水中。搅拌直至充分混合，然后加水至终体积 1L。在 2-8°C 下最多保存一周。
相关产品
产品编号
说明
价格
WGK
WGK 1
法规信息
监管及禁止进口产品
Tadatsura Koshika et al.
Immunology, 134(4), 386-397 (2011-11-03)
α1,3-Galactosyltransferase gene-knockout pigs transgenic for porcine cytotoxic T-lymphocyte antigen 4 immunoglobulin (pCTLA4-Ig) have been produced to reduce T-cell-mediated rejection following xenotransplantation. The level of soluble pCTLA4-Ig in their blood was greatly in excess of the therapeutic level in patients, rendering
Serapion Pyrpassopoulos et al.
Methods in molecular biology (Clifton, N.J.), 1046, 389-403 (2013-07-23)
Many proteins that reside in the cytoplasm bind directly to cell membranes and play roles in signaling, adhesion, metabolism, cell structure, and cell motility. Several of these membrane-binding proteins, especially cytoskeletal proteins, have mechanical functions that result in the transmission
Ochratoxin A in some French wines: application of a direct competitive ELISA based on an OTA-HRP conjugate.
Radoi, A., et al.
Analytical Letters, 42, 1187-1187 (2009)
Elizabeth R Sunderhaus et al.
Frontiers in neuroscience, 13, 1207-1207 (2019-11-30)
Mutations in patatin-like phospholipase domain-containing protein 6 (PNPLA6) have been linked with a number of inherited diseases with clinical symptoms that include spastic paraplegia, ataxia, and chorioretinal dystrophy. PNPLA6 is an evolutionary conserved protein whose ortholog in Drosophila is Swiss-Cheese
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
实验方案
Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
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