一般描述
酪蛋白是存在于乳汁中的磷蛋白。它分为四种亚型，包括αS1-、αS2-、β-和 κ-酪蛋白。
Sigma 的 10x 封闭缓冲液是一种基于酪蛋白的封闭剂，常规用于减少检测试剂与 Northern 和 Southern 印迹膜的非特异性结合。
Sigma 的 10x 封闭缓冲液是一种基于酪蛋白的封闭剂，常规用于减少检测试剂与 Northern 和 Southern 印迹膜的非特异性结合。
应用
酪蛋白封闭缓冲液10X已用于：
- 蛋白印迹
- 酶联免疫吸附分析(ELISA)
- 免疫组化
特点和优势
- 封闭性能优于奶粉或 BSA
- 易于使用的浓缩液
- 与中性和正电荷膜兼容
- 与化学发光和比色检测试剂盒兼容
制备说明
使用前用分子生物学级水稀释 10x 封闭缓冲液。
警示用语：
Warning
危险分类
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
含少量动物源组分生物产品
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实验方案
Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
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