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Merck
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文件

安全信息

B6429

Sigma-Aldrich

酪蛋白封闭缓冲液 10x

for Northern and Southern blotting, solution

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别名:
10x Blocking Buffer
UNSPSC代码:
12352200
NACRES:
NA.54

等级

for molecular biology

质量水平

200

形式

solution

技术

Southern blotting: suitable

储存温度

room temp

相关类别

一般描述

酪蛋白是存在于乳汁中的磷蛋白。它分为四种亚型，包括αS1-、αS2-、β-和 κ-酪蛋白。
Sigma 的 10x 封闭缓冲液是一种基于酪蛋白的封闭剂，常规用于减少检测试剂与 Northern 和 Southern 印迹膜的非特异性结合。

应用

酪蛋白封闭缓冲液10X已用于：

  • 蛋白印迹
  • 酶联免疫吸附分析(ELISA)
  • 免疫组化

特点和优势

  • 封闭性能优于奶粉或 BSA
  • 易于使用的浓缩液
  • 与中性和正电荷膜兼容
  • 与化学发光和比色检测试剂盒兼容

制备说明

使用前用分子生物学级水稀释 10x 封闭缓冲液。

危险分类

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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