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HomeLarge Molecule HPLCHPLC Analysis of Immunoglobulin G (IgG) on Zenix® SEC-150 versus Zenix® SEC-300, Effect of Pore Size on Resolution

HPLC Analysis of Immunoglobulin G (IgG) on Zenix® SEC-150 versus Zenix® SEC-300, Effect of Pore Size on Resolution

Graphh Zenix SEC-150 & SEC-300

Conditions

Column   Zenix SEC-150, 30 cm x 4.6 mm I.D., 3 µm, 150 Angstrom particles (Z777016)
Column   Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 µm, 300 Angstrom particles (Z777028)
mobile phase   150 mM sodium phosphate, pH 7.0
Flow rate   1 mL/min
Sample   1 mg/mL, IgG
Injection   10 µL
Detector   UV, 214 nm

Description

Categories Analytical Chromatography, Antibiotics, Antimicrobials
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
Suitability application for HPLC
MATERIALS
Product No.Product NameDescriptionPricing
5.33001Acetic acid 100%for LC-MS LiChropur™
1.00029Acetonitrilehypergrade for LC-MS LiChrosolv®
73594Ammonium acetateLiChropur™, eluent additive for LC-MS
70221Ammonium formateeluent additive for LC-MS, LiChropur™, ≥99.0%
1.11727Ethanolgradient grade for liquid chromatography LiChrosolv®
5.33002Formic acid 98% - 100%for LC-MS LiChropur™
56834Immunoglobulin G from human serum≥95% (GE)
1.06007Methanolgradient grade for liquid chromatography LiChrosolv® Reag. Ph Eur
1.06035Methanolhypergrade for LC-MS LiChrosolv®
5.43841Potassium phosphate monobasicfor HPLC LiChropur™
342483Sodium phosphate96%
69372Triethylammonium acetate buffersuitable for HPLC, 0.98-1.02 M
1.15333Waterfor chromatography (LC-MS Grade) LiChrosolv®
Z777016Zenix® SEC-150 Gel Filtration Column, 3 μmL × I.D. 30 cm × 4.6 mm, 3 μm particle size, stainless steel
Z777028Zenix® SEC-300 Gel Filtration Column, 3 μmL × I.D. 30 cm × 4.6 mm, 3 μm particle size, stainless steel
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