Home Large Molecule HPLCHPLC Analysis of Immunoglobulin G (IgG) on Zenix^® SEC-150 versus Zenix^® SEC-300, Effect of Pore Size on Resolution

HPLC Analysis of Immunoglobulin G (IgG) on Zenix^® SEC-150 versus Zenix^® SEC-300, Effect of Pore Size on Resolution

Conditions
Column	Zenix SEC-150, 30 cm x 4.6 mm I.D., 3 µm, 150 Angstrom particles (Z777016)
Column	Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 µm, 300 Angstrom particles (Z777028)
mobile phase	150 mM sodium phosphate, pH 7.0
Flow rate	1 mL/min
Sample	1 mg/mL, IgG
Injection	10 µL
Detector	UV, 214 nm

Description
Categories	Analytical Chromatography, Antibiotics, Antimicrobials
Featured Industry	Life Science and Biopharma
Legal Information	Zenix is a registered trademark of Sepax Technologies
Suitability	application for HPLC

MATERIALS

Product Number	Product Name	Product Description
5.33001	Acetic acid 100%	for LC-MS LiChropur^™
1.00029	Acetonitrile	hypergrade for LC-MS LiChrosolv^®
73594	Ammonium acetate	LiChropur^™, eluent additive for LC-MS
70221	Ammonium formate	eluent additive for LC-MS, LiChropur^™, ≥99.0%
1.11727	Ethanol	gradient grade for liquid chromatography LiChrosolv^®
5.33002	Formic acid 98% - 100%	for LC-MS LiChropur^™
56834	Immunoglobulin G from human serum	≥95% (GE)
1.06007	Methanol	gradient grade for liquid chromatography LiChrosolv^® Reag. Ph Eur
1.06035	Methanol	hypergrade for LC-MS LiChrosolv^®
5.43841	Potassium phosphate monobasic	for HPLC LiChropur^™
342483	Sodium phosphate	96%
69372	Triethylammonium acetate buffer	suitable for HPLC, 0.98-1.02 M
1.15333	Water	for chromatography (LC-MS Grade) LiChrosolv^®
Z777016	Zenix^® SEC-150 Gel Filtration Column, 3 μm	L × I.D. 30 cm × 4.6 mm, 3 μm particle size, stainless steel
Z777028	Zenix^® SEC-300 Gel Filtration Column, 3 μm	L × I.D. 30 cm × 4.6 mm, 3 μm particle size, stainless steel

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HPLC Analysis of Immunoglobulin G (IgG) on Zenix® SEC-150 versus Zenix® SEC-300, Effect of Pore Size on Resolution