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column	TSKgel Bio Assist S, 5 cm x 4.6 mm I.D., 7 µM particles (819686)
column	Competitive S-type column, 5 cm x 5 mm I.D.
column temp.	25 °C
mobile phase	[A] 20 mM sodium phophate buffer, pH 7.0; [B] 20 mM sodium phophate buffer, 0.5 M NaCl, pH 7.0
gradient	0 to 100% B in 20 min (0 to 100% B in 30 min for competitive column)
flow rate	0.8 mL/min (1.0 mL/min for competitive column)
sample	crude bromelain, 0.01 µg/mL
detector	UV, 280 nm

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Categories	Analytical Chromatography, Proteins
Featured Industry	Life Science and Biopharma
Legal Information	BioAssist is a registered trademark of Tosoh Corporation, TSKgel is a registered trademark of Tosoh Corporation
Other Notes	Data provided by Tosoh Bioscience LLC.
suitability	application for HPLC

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