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LC/MS Analysis of Illicit Bath Salts on Ascentis® Express HILIC, Set 4

LC/MS Analysis of Illicit Bath Salts on Ascentis® Express HILIC, Set 4 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Express HILIC, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

Application

Product No.
Description
Pricing

LC/MS Analysis of Illicit Bath Salts on Ascentis® Express HILIC, Set 1

application for HPLC

LC/MS Analysis of Illicit Bath Salts on Ascentis® Express HILIC, Set 2

application for HPLC

LC/MS Analysis of Illicit Bath Salts on Ascentis® Express HILIC, Set 3

application for HPLC

standard

Product No.
Description
Pricing

Buphedrone hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

Mephedrone hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

Methylone hydrochloride

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

MDAI hydrochloride solution

100 μg/mL in acetonitrile: water (9:1) with 5% 1 M HCl (as free base), ampule of 1 mL, certified reference material, Cerilliant®

Pyrovalerone hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

N-Ethylcathinone hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)

mobile phase

5 mM ammonium formate in [A] acetonitrile; [B] water; (98:2, A:B)

flow rate

0.6 mL/min

pressure

267 psi (18.4 bar)

column temp.

35 °C

detector

MS, ESI(+) TOF, Summed Ion Chromatogram

injection

2 μL

sample

500 ng/mL in acetonitrile

Description

Analysis Note

HILIC mode operation on an Ascentis Express column provided baseline resolution of these bath salts, including isobaric compounds. Ultra high purity solvents were used to supply low background interference and low particulate contaminants for robust; trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany