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HPLC Analysis of Polar Compounds on Ascentis® Express OH5 (HILIC Mode)

HPLC Analysis of Polar Compounds on Ascentis® Express OH5 (HILIC Mode) application for HPLC

Materials

analytical column

Product No.
Description
Pricing

53769-U

Ascentis® Express OH5, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

standard

Product No.
Description
Pricing

C1778

Caffeine

Sigma Reference Standard, vial of 250 mg

C5901

Codeine monohydrate

analytical standard

CONDITIONS

column

Ascentis Express OH5, 10 cm x 3 mm I.D., 2.7 μm particles (53769-U)

mobile phase

5 mM ammonium formate (95:5 acetonitrile:water) pH 6.8

flow rate

0.6 mL/min

pressure

965 psi (66.5 bar)

column temp.

30 °C

detector

UV, 254 nm

injection

1 μL

sample

100 μg/mL in 25:75, water:methanol

Description

Analysis Note

The impact of partitioning and ion exchange in HILIC separations is demonstrated using a mixture of acidic, neutral, and basic polar compounds in this Figure. All three analytes have similar log D values, both acid and base are ionized at mobile phase pH.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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