HPLC Analysis of Oxazepam Enantiomers on Astec^® CYCLOBOND^™ I 2000 DNP

column

CYCLOBOND I 2000 DNP, 25 cm x 4.6 mm I.D., 5 μm particles (25024AST)

mobile phase

[A] acetonitrile; [B] 20 mM ammonium phosphate, pH 2.9; (20:80, A:B)

flow rate

1 mL/min

column temp.

5 °C

detector

UV, 254 nm

injection

3 μL

sample

oxazepam, 1 mg/mL in acetonitrile:water (50:50)