HPLC Analysis of Fenofibric Acid Enantiomers on Astec^® CYCLOBOND^™ I 2000

column

CYCLOBOND I 2000, 25 cm x 4.6 mm I.D., 5 μm particles (20024AST)

mobile phase

[A] 0.1% triethylamine acetate, pH 4.0; [B] acetonitrile; (20:80, A:B)

flow rate

1 mL/min

column temp.

22 °C

detector

UV, 254 nm

injection

1 μL

sample

3 mg/mL in methanol