HPLC Analysis of Basic Peptides (Angiotensin Analogs) on BIOshell A160 Peptide C18
Materials
analytical column
Product No.
Description
Pricing
Ascentis® Express Peptide ES-C18, 2.7 μm HPLC Column
2.7 μm particle size, L × I.D. 10 cm × 3 mmCONDITIONS
column
BIOshell A160 Peptide C18, 10 cm x 3 mm I.D., 2.7 μm particles (66908-U)
mobile phase
[A] 0.1% (v/v) formic acid, pH 4.0 (titrated with ammonium hydroxide); [B] 50:50, (0.175% formic acid in water, pH 4.0) : acetonitrile
gradient
20 to 50% B in 15 min
flow rate
0.6 mL/min
pressure
3002 psi (207 bar)
column temp.
35 °C
detector
UV, 215 nm
injection
2.0 μL
sample
0.5 g/L ea. peptide
Description
Legal Information
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