HPLC Analysis of Alloisoleucine Enantiomers on Astec^® CHIROBIOTIC^® R

column

CHIROBIOTIC R, 25 cm x 4.6 mm I.D., 5 μm particles (13024AST)

mobile phase

[A] water; [B] methanol; (40:60, A:B)

flow rate

0.6 mL/min

column temp.

25 °C

detector

UV, 210 nm

injection

10 μL

sample

1 mg/mL in methanol