HPLC Analysis of a Monoclonal Antibody on Zenix® SEC-300 after Partial Proteolysis with IdeS Protease
Materials
related product
Product No.
Description
Pricing
CONDITIONS
column
Zenix SEC-300, 30 cm X 4.6 mm I.D., 3 μm particles (Z777087)
mobile phase
0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide
flow rate
0.35 mL/min
pressure
1350 psi (93 bar)
column temp.
ambient
detector
UV, 215 nm
injection
1 μL
sample
digested SO57 mAb, ~0.5 g/L
Description
Analysis Note
Proteolysis of antibodies can be useful for simplifying further analysis. This application demonstrates separation of the two fragments, F(ab′)2 and scFc from the intact IgG, after partial digestion with IdeS protease.
Legal Information
Zenix is a registered trademark of Sepax Technologies