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HPLC Analysis of a Monoclonal Antibody on Zenix®-C SEC-150 after Partial Proteolysis with IdeS Protease

HPLC Analysis of a Monoclonal Antibody on Zenix®-C SEC-150 after Partial Proteolysis with IdeS Protease application for HPLC

Materials

related product

Product No.
Description
Pricing

07298

FabRICATOR®

for cleaving 2 mg IgG, 2000 U/vial

CONDITIONS

column

Zenix-C SEC-150, 30 cm X 4.6 mm I.D., 3 μm particles (Z777083)

mobile phase

0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide (567329-U)

flow rate

0.35 mL/min

pressure

1450 psi (100 bar)

column temp.

ambient

detector

UV, 215 nm

injection

1 μL

sample

digested SO57 mAb, ~0.5 g/L

Description

Analysis Note

Proteolysis of antibodies can be useful for simplifying further analysis. This application demonstrates separation of the two fragments, F(ab′)2 and scFc from the intact IgG, after partial digestion with IdeS protease.

Legal Information

Zenix is a registered trademark of Sepax Technologies

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS