HPLC Analysis of 2-Phenylpropionic Acid Enantiomers on Astec® CYCLOBOND™ I 2000 HP-RSP
Materials
analytical column
Product No.
Description
Pricing
Astec® CYCLOBOND™ I 2000 HP-RSP Chiral HPLC Column
5 μm particle size, L × I.D. 25 cm × 4.6 mmCONDITIONS
column
CYCLOBOND I 2000 HP-RSP, 25 cm x 4.6 mm I.D., 5 μm particles (24024AST)
mobile phase
[A] 10 mM ammonium acetate, pH 4.0; [B] acetonitrile; (75:25, A:B)
flow rate
1 mL/min
column temp.
25 °C
detector
UV, 235 nm
injection
1 μL
sample
1 mg/mL in methanol
Description
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CYCLOBOND is a trademark of Sigma-Aldrich Co. LLC