HPLC Analysis of 2,2′-Bis[(S)-(4-methylphenyl)sulfinyl]-1,1′-binapthaIene Diastereomers on Ascentis^® Express C18

column

Ascentis Express C18, 10 cm x 4.6 mm I.D., 2.7 μm particles (53827-U)

mobile phase

[A] water; [B] methanol; (25:75, A:B)

flow rate

0.8 mL/min

pressure

2450 psi (169 bar)

column temp.

35 °C

detector

UV, 240 nm

injection

5 μL

sample

(1R)-2,2′-Bis[(S )-(4-methylphenyl)sulfonyl]-1,1′-binapthalene, 25 μg/mL in methanol; (1S)-2,2′-Bis[(S)-(4-methylphenyl)sulfonyl]-1,1′-binapthalene, 50 μg/mL in methanol