HPLC Analysis of 16 Triazine and Phenyl Urea Compounds on Ascentis® Express RP-Amide, Modified Gradient
Materials
analytical column
Product No.
Description
Pricing
CONDITIONS
column
Ascentis Express RP-Amide, 15 cm x 3 mm I.D., 2.7 μm particles (53919-U)
mobile phase
[A] water; [B] acetonitrile
gradient
10 to 25% B in 30 min; 25 to 60% B in 15 min; held at 60% B for 5 min
flow rate
0.4 mL/min
column temp.
35 °C
detector
UV, 240 nm
injection
5 μL
sample
20 mg/L each (except terbuthylazin desethyl and terbuthylazine 60 mg/L) in 60:40, water: acetonitrile
Description
Analysis Note
This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of 16 triazine and phenyl urea compounds. Lower column
temperature can improve resolution of the critical pair.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany