GC Analysis of Volatiles in Multifloral Honey on SUPELCOWAX™ 10 after SPME using a 50/30 μm DVB/Carboxen/PDMS Fiber
Materials
analytical column
SPME fiber
SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS)
needle size 24 ga, StableFlexrelated product
standard
CONDITIONS
sample/matrix
5 g multifloral honey + 5 mL bidistilled water in a 20 mL vial, allowed to equilibrate at 50 °C for 15 min with magnetic stirring
SPME fiber
2 cm 50/30 μm divinylbenzene/carboxen on polydimethylsiloxane (57348-U)
extraction
headspace, 50 °C for 40 min with magnetic stirring
desorption process
250 °C for 2 min
column
SUPELCOWAX 10, 30 m x 0.25 mm I.D., 0.25 μm (24079)
oven
35 °C (8 min), 6 °C/min to 60 °C, 4 °C/min to 160 °C, 20 °C/min to 200 °C (1 min)
inj. temp.
250 °C
MSD interface
230 °C
scan range
35-300 m/z
carrier gas
helium, 1 mL/min
liner
0.75 mm I.D., SPME
sample
(Honey)
Description
Analysis Note
In this application, headspace SPME (HS-SPME) using a Carboxen/PDMS/DVB fiber coupled with gas chromatography-mass spectrometry (GC/MS) was used for the characterization of the volatile fraction of honey samples. Using SPME it was possible to obtain and distinguish the chemical fingerprints of different types of honey. The relatively polar SUPELCOWAX 10 GC column provides excellent separation of these polar compounds.
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