Non-conjugated cis/trans 18:2 in Beef Fat are Mainly Δ-9 Desaturation Products of trans-18:1 Isomers.

Non-conjugated cis/trans 18:2 in Beef Fat are Mainly Δ-9 Desaturation Products of trans-18:1 Isomers.

Lipids (2016-11-18)

P Vahmani, D C Rolland, K E Gzyl, M E R Dugan

ABSTRACT

Human liver cells (HepG2) were cultured with individual trans (t) 18:1 including t6-, t12-, t13-, t14-, t15- and t16-18:1, and retention times of their Δ-9 desaturation products were determined using 100-m biscyanopropyl-polysiloxane and SLB-IL111 columns. Corresponding peaks were found in beef adipose tissues known to have different delta-9 desaturase activities. Further lines of evidence indicating the presence of Δ-9 desaturation products of t-18:1 isomers in beef fat were developed by analysis of fatty acid methyl esters (FAME) fractionated using Ag

MATERIALS

Product Number

Brand

Product Description

29734-U

Supelco

SLB^®-1ms Capillary GC Column, L × I.D. 30 m × 0.32 mm, d_f 0.10 μm

29735-U

Supelco

SLB^®-1ms Capillary GC Column, L × I.D. 15 m × 0.32 mm, d_f 0.25 μm

29737-U

Supelco

SLB^®-1ms Capillary GC Column, L × I.D. 60 m × 0.32 mm, d_f 0.25 μm