SMAD3 Regulates Follicle-stimulating Hormone Synthesis by Pituitary Gonadotrope Cells

SMAD3 Regulates Follicle-stimulating Hormone Synthesis by Pituitary Gonadotrope Cells

The Journal of biological chemistry (2016-12-21)

Yining Li, Gauthier Schang, Ulrich Boehm, Chu-Xia Deng, Jonathan Graff, Daniel J Bernard

ABSTRACT

Pituitary follicle-stimulating hormone (FSH) is an essential regulator of fertility in females and of quantitatively normal spermatogenesis in males. Pituitary-derived activins are thought to act as major stimulators of FSH synthesis by gonadotrope cells.

MATERIALS

Product Number

Brand

Product Description

T48402

Sigma-Aldrich

2,2,2-Tribromoethanol, 97%

P6148

Sigma-Aldrich

Paraformaldehyde, reagent grade, crystalline

H3884

Sigma-Aldrich

Hyaluronidase from bovine testes, Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid

M7653

Sigma-Aldrich

Medium 199, With Hanks′ salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture