- Endothelial cell-derived pentraxin 3 limits the vasoreparative therapeutic potential of circulating angiogenic cells.
Endothelial cell-derived pentraxin 3 limits the vasoreparative therapeutic potential of circulating angiogenic cells.
Cardiovascular research (2016-09-24)
Christina L O'Neill, Jasenka Guduric-Fuchs, Sarah E J Chambers, Michelle O'Doherty, Barbara Bottazzi, Alan W Stitt, Reinhold J Medina
PMID27659714
ABSTRACT
Circulating angiogenic cells (CACs) promote revascularization of ischaemic tissues although their underlying mechanism of action and the consequences of delivering varying number of these cells for therapy remain unknown. This study investigates molecular mechanisms underpinning CAC modulation of blood vessel formation. CACs at low (2 × 10 Endothelium, when exposed to HD CACs, releases PTX3 which markedly impairs the vascular regenerative response in an autocrine manner. Therefore, CAC density and accompanying release of angiocrine PTX3 are critical considerations when using these cells as a cell therapy for ischaemic disease.
MATERIALS