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  • A mechanism-based affinity-labeling agent for possible use in isolating N-acetylglucosaminidase.

A mechanism-based affinity-labeling agent for possible use in isolating N-acetylglucosaminidase.

Bioorganic & medicinal chemistry letters (2001-06-27)
M Ichikawa, Y Ichikawa
ABSTRACT

We have prepared several mechanism-based affinity-labeling agents for possible use in isolating N-acetylglucosaminidase, in which an N-acetylglucosamine is linked to an o-monofluoro- or difluoro-methyl phenoxy glycoside with or without a cleavable disulfide group in the tether to biotin.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Biotin disulfide N-hydroxysuccinimide ester, >98%, powder
Sigma-Aldrich
Sulfo-NHS-LC-LC-Biotin, ≥95%