- Specific association of lectin LecB with the surface of Pseudomonas aeruginosa: role of outer membrane protein OprF.
Specific association of lectin LecB with the surface of Pseudomonas aeruginosa: role of outer membrane protein OprF.
PloS one (2012-10-12)
Horst Funken, Kai-Malte Bartels, Susanne Wilhelm, Melanie Brocker, Michael Bott, Manjeet Bains, Robert E W Hancock, Frank Rosenau, Karl-Erich Jaeger
PMID23056489
ABSTRACT
The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we report that LecB binds to the outer membrane protein OprF. In an OprF-deficient P. aeruginosa mutant, LecB is no longer detectable in the membrane but instead in the culture supernatant indicating a specific interaction between LecB and OprF.
MATERIALS