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  • Multi-method approach for characterizing the interaction between Fusarium verticillioides and Bacillus thuringiensis subsp. Kurstaki.

Multi-method approach for characterizing the interaction between Fusarium verticillioides and Bacillus thuringiensis subsp. Kurstaki.

PloS one (2014-04-18)
Liliana O Rocha, Sabina Moser Tralamazza, Gabriela M Reis, Leon Rabinovitch, Cynara B Barbosa, Benedito Corrêa
ABSTRACT

Bacterial antagonists used as biocontrol agents represent part of an integrated management program to reduce pesticides in the environment. Bacillus thuringiensis is considered a good alternative as a biocontrol agent for suppressing plant pathogens such as Fusarium. In this study, we used microscopy, flow cytometry, indirect immunofluorescence, and high performance liquid chromatography to determine the interaction between B. thuringiensis subsp. kurstaki LFB-FIOCRUZ (CCGB) 257 and F. verticillioides MRC 826, an important plant pathogen frequently associated with maize. B. thuringiensis showed a strong in vitro suppressive effect on F. verticillioides growth and inhibited fumonisin production. Flow cytometry analysis was found to be adequate for characterizing the fungal cell oscillations and death during these interactions. Further studies of the antagonistic effect of this isolate against other fungi and in vivo testing are necessary to determine the efficacy of B. thuringiensis subsp. kurstaki in controlling plant pathogens. This is the first report on the use of flow cytometry for quantifying living and apoptotic F. verticillioides cells and the B. thuringiensis Cry 1Ab toxin.

MATERIALS
Product Number
Brand
Product Description

Supelco
Propyl gallate, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Propyl gallate, powder
Sigma-Aldrich
Propyl gallate, for microscopy, ≥98.0% (HPLC)
Sigma-Aldrich
Propyl gallate, antioxidant, ≥98.0% (HPLC)
Sigma-Aldrich
Propyl gallate, ≥98%, FCC
USP
Propyl gallate, United States Pharmacopeia (USP) Reference Standard