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  • A ferrofluid-based homogeneous assay for highly sensitive and selective detection of single-nucleotide polymorphisms.

A ferrofluid-based homogeneous assay for highly sensitive and selective detection of single-nucleotide polymorphisms.

Chemical communications (Cambridge, England) (2013-08-08)
Wei Shen, Cai Le Lim, Zhiqiang Gao
ABSTRACT

A simple and low-cost colorimetric assay utilizing ferrofluidic nanoparticulate probes (FNPs) and a ligase for single-nucleotide polymorphism genotyping is described. Excellent sensitivity and selectivity were accomplished through the engagement of the FNPs and a ligase chain reaction.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Tetramethylammonium chloride, BioUltra, for molecular biology, ≥99.0% (AT)
Supelco
Tetramethylammonium sulfate, suitable for ion pair chromatography, LiChropur, ≥99.0% (T)
Supelco
Tetramethylammonium chloride, suitable for ion pair chromatography, LiChropur, ≥99.0% (AT)
Sigma-Aldrich
Tetramethylammonium chloride, reagent grade, ≥98%
Sigma-Aldrich
Tetramethylammonium hydroxide pentahydrate, ≥95.0% (T)
Sigma-Aldrich
Tetramethylammonium iodide, 99%
Sigma-Aldrich
Tetramethylammonium chloride solution, for molecular biology
Sigma-Aldrich
Tetramethylammonium hydroxide pentahydrate, ≥97%