"Cluster-ion" bombardment of biological macromolecules: Morphological evidence for disassembly of single tobacco mosaic virus particles.

"Cluster-ion" bombardment of biological macromolecules: Morphological evidence for disassembly of single tobacco mosaic virus particles.

Proceedings of the National Academy of Sciences of the United States of America (1987-01-01)

M C Ledbetter, R J Beuhler, L Friedman

ABSTRACT

Cluster ions containing 50, 100, or 200 water molecules and one proton are accelerated to over 275 kV and impacted on thin carbon films on which tobacco mosaic virus has been dispersed. After bombardment the films are examined with a transmission electron microscope to study induced morphological alterations with respect to the energy transfer processes that take place during and after single cluster-ion impact. Observations on tobacco mosaic virus indicate that a single cluster impact results in the removal of a cylindrical segment of the virus. No fragments from the excised TMV segment were observed on the carbon foil. We speculate that cluster-ion bombardment may become a useful analytical technique for structural studies of large biological macromolecular complexes.

MATERIALS

Product Number

Brand

Product Description

GF37642822

Carbon, foil, 500x500mm, thickness 0.35mm, flexible graphite, 99.8%

GF91624103

Carbon, foil, 12mm disks, thickness 1.0mm, rigid graphite, fine grain size, 99.95%

GF26559513

Carbon, foil, 150x150mm, thickness 5.0mm, rigid graphite, medium grain size, 99.5%

GF97410708

Carbon, foil, 250x250mm, thickness 1.0mm, flexible graphite, 99.8%

GF14397521

Carbon, foil, 25x25mm, thickness 1.0mm, rigid graphite, fine grain size, 99.95%

GF29446814

Carbon, foil, 500x500mm, thickness 0.5mm, flexible graphite, 99.8%

GF60685656

Carbon, foil, 25x25mm, thickness 0.125mm, rigid graphite, fine grain size, 99.95%

GF46425078

Carbon, foil, 50x50mm, thickness 0.2mm, pyrolytic graphite, 99.99%

GF77841906

Carbon, foil, not light tested, 300x300mm, thickness 0.125mm, flexible graphite, 99.8%

GF13787332

Carbon, foil, 50x50mm, thickness 1.0mm, flexible graphite, 99.8%

GF64201249

Carbon, foil, 25x25mm, thickness 2.5mm, rigid graphite, fine grain size, 99.95%

GF71469939

Carbon, foil, 5x5mm, thickness 2.0mm, hOpg

GF31912039

Carbon, foil, 150x150mm, thickness 0.5mm, rigid graphite, fine grain size, 99.95%

GF57718503

Carbon, foil, 25x25mm, thickness 0.15mm, rigid graphite, fine grain size, 99.95%

GF01171763

Carbon, foil, 50x50mm, thickness 0.35mm, flexible graphite, 99.8%

GF23996290

Carbon, foil, 300x300mm, thickness 0.2mm, flexible graphite, 99.8%

GF64932947

Carbon, foil, 10mm disks, thickness 0.2mm, flexible graphite, 99.8%

GF27179286

Carbon, foil, 150x150mm, thickness 1.0mm, flexible graphite, 99.8%

GF71541031

Carbon, foil, not light tested, 150x150mm, thickness 0.075mm, flexible graphite, 99.8%

GF87162857

Carbon, foil, 50x50mm, thickness 0.5mm, flexible graphite, 99.8%

GF11412470

Carbon, foil, 100x100mm, thickness 5.0mm, rigid graphite, medium grain size, 99.5%

GF99536338

Carbon, foil, 500x500mm, thickness 0.2mm, flexible graphite, 99.8%

GF83177949

Carbon, foil, 12mm disks, thickness 0.5mm, rigid graphite, fine grain size, 99.95%

GF82985844

Carbon, foil, 150x150mm, thickness 1.0mm, rigid graphite, fine grain size, 99.95%

GF34902109

Carbon, foil, 10x10mm, thickness 2.0mm, hOpg

GF42491291

Carbon, foil, 50x50mm, thickness 5.0mm, rigid graphite, medium grain size, 99.5%

GF55242558

Carbon, foil, 6mm disks, thickness 0.2mm, flexible graphite, 99.8%

GF58259469

Carbon, foil, 50x50mm, thickness 0.2mm, flexible graphite, 99.8%

GF02861782

Carbon, foil, not light tested, 300x300mm, thickness 0.075mm, flexible graphite, 99.8%

GF27136100

Carbon, foil, 25x25mm, thickness 0.25mm, rigid graphite, fine grain size, 99.997%