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  • Isolation and characterization of a thermophilic bacterium, Geobacillus thermocatenulatus, degrading nylon 12 and nylon 66.

Isolation and characterization of a thermophilic bacterium, Geobacillus thermocatenulatus, degrading nylon 12 and nylon 66.

Biotechnology letters (2003-11-25)
Kosuke Tomita, Noritoshi Ikeda, Ayumi Ueno
ABSTRACT

A thermophilic bacterium, identified as a neighboring species to Geobacillus thermocatenulatus, having a growth optimum at 55 degrees C and, capable of degrading nylon 12, was isolated from soil by enrichment culture technique at 60 degrees C. At this temperature, the strain grew on 5 g nylon 12 l(-1) with a decrease in its molecular weight from 41000 to 11000 over 20 d. The degradation was assumed to be due to endogenous hydrolysis of amide bond in nylon 12. The strain degraded also nylon 66 with a decrease in its molecular weight from 43000 to 17000 in 20 d at 60 degrees C. Nylon 6 was not degraded.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Nylon 12, pellets