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  • A rapid method for the synthesis of methylmalonyl-coenzyme A and other CoA-esters.

A rapid method for the synthesis of methylmalonyl-coenzyme A and other CoA-esters.

Analytical biochemistry (1993-10-01)
R Padmakumar, S Gantla, R Banerjee
ABSTRACT

A rapid and high-yield synthesis of methylmalonyl coenzyme A is reported. The two-step procedure involves preparation of the thiophenyl ester of methylmalonic acid using dicyclohexylcarbodiimide as a condensing agent, followed by transesterification with coenzyme A, to yield methylmalonyl coenzyme A in 80% overall yield. Inclusion of an additional step, methylation of malonic acid with iodomethane, affords the opportunity for introducing a stable or radioactive isotope into the product. This method should be applicable for the syntheses of other coenzyme A esters that are of biochemical interest such as succinyl coenzyme A.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Dimethyl malonate, 98%
Sigma-Aldrich
Dimethyl malonate, purum, ≥96.0% (GC)