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  • Degradation pattern of gibberellic acid during the whole process of tea production.

Degradation pattern of gibberellic acid during the whole process of tea production.

Food chemistry (2013-02-16)
Hongping Chen, Xin Liu, Dan Yang, Peng Yin
ABSTRACT

The residues of gibberellic acid (GA(3)) in tea shoots, made tea, and tea infusion were determined by ultra-performance liquid chromatography tandem mass (UPLC-MS/MS) to study its degradation pattern during tea planting, processing, and brewing. The dissipation rate of GA(3) was described using first-order kinetics. Its half-life ranged from 1.67 to 2.01 days in tea shoots. Degradation and concentration during green tea processing had equally important functions on GA(3) residues in product intermediates and made tea. Except for water content, little GA(3) residue difference was found in tea shoots and made tea. GA(3) dissipated rapidly in the baking stage during processing. The transfer coefficient of GA(3) residues from made tea to infusion was from 26.23% to 54.55%. GA(3) extraction efficiency varied with different infusion times and concentrations of GA(3) in made tea. This research revealed that GA(3) may be safe when applied in tea gardens at suitable doses and picking intervals.

MATERIALS
Product Number
Brand
Product Description

Supelco
Gibberellic acid, PESTANAL®, analytical standard
Sigma-Aldrich
Gibberellic acid, 90% gibberellin A3 basis (HPLC)
Sigma-Aldrich
Gibberellic acid, suitable for plant cell culture, BioReagent, ≥90% gibberellin A3 basis (of total gibberellins.)
Sigma-Aldrich
Gibberellic acid potassium salt, suitable for plant cell culture, BioReagent, ~95%, ≥50% total GA3 basis