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  • Studies of inhibitor binding to the [4Fe-4S] cluster of quinolinate synthase.

Studies of inhibitor binding to the [4Fe-4S] cluster of quinolinate synthase.

Angewandte Chemie (International ed. in English) (2012-06-21)
Alice Chan, Martin Clémancey, Jean-Marie Mouesca, Patricia Amara, Olivier Hamelin, Jean-Marc Latour, Sandrine Ollagnier de Choudens
ABSTRACT

Stop for NadA! A [4Fe-4S] enzyme, NadA, catalyzes the formation of quinolinic acid in de novo nicotinamide adenine dinucleotide (NAD) biosynthesis. A structural analogue of an intermediate, 4,5-dithiohydroxyphthalic acid (DTHPA), has an in vivo NAD biosynthesis inhibiting activity in E. coli. The inhibitory effect can be explained by the coordination of DTHPA thiolate groups to a unique Fe site of the NadA [4Fe-4S] cluster.

MATERIALS
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Brand
Product Description

Sigma-Aldrich
Dihydroxyacetone phosphate hemimagnesium salt hydrate, ≥95% (TLC)