[Cloning and expression of prenyl transferase gene of Metarhizium ansopliae].

To clone and identify prenyl transferase gene from Metarhizium anisopliae and to understand the gene structure and expression. Using Switching Mechanism At 5' end of RNA Transcript (SMART) method, we isolated the full length cDNA sequence and DNA sequence. Then we used quantitative RT-PCR analysis of the gene expression levels at different stages of colonization of host hemolymph by M. anisopliae. The Mpt gene had two exons and one intron and the CDS was 1026 bp which encoded a protein with 341 amino acid residues; qRT-PCR analysis showed that the gene expression levels were significantly different, especially highly up-regulated at the late stages. The Mpt gene was successfully cloned from M. anisopliae for the first time and the gene had the characteristic of high expression levels at the late stages.