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  • [Effect of triterpenoid glycosides on alpha- and beta-amylase activity and total protein content in wheat seedlings].

[Effect of triterpenoid glycosides on alpha- and beta-amylase activity and total protein content in wheat seedlings].

Prikladnaia biokhimiia i mikrobiologiia (2012-01-12)
E S Davidiants
ABSTRACT

Influence of the aleanolic acid glycosides from Silphium perfoliatum L. (silphioside B, C, E and G) and their progenins on the amylase activity and total protein content in wheat seedlings was studied. Treatment of the Triticum aestivum L. seeds with 1-10 microM water solutions of mono- and diglycosides (mono- and bisdesmosines) elevated the alpha-amylase and total amylase activities in seedlings. Silphioside E containing three glucose moieties in its molecule did not change alpha-amylase activity, but it did if bis-triglycoside acetylated carbohydrate (as in silphioside C). Effects of 5-10 microM solutions of the active glycosides was comparable with that of exogenous gibberellin A3 and 6-benzylaminopurine.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
6-Benzylaminopurine, ReagentPlus®, ≥99.0% (HPLC)
Sigma-Aldrich
6-Benzylaminopurine, suitable for plant cell culture
Sigma-Aldrich
β-Amylase from barley, Type II-B, 20-80 units/mg protein (biuret)
Sigma-Aldrich
6-Benzylaminopurine, Vetec, reagent grade, 98%
Supelco
6-Benzylaminopurine, PESTANAL®, analytical standard
Sigma-Aldrich
β-Amylase from sweet potato, Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280)
Sigma-Aldrich
β-Amylase from sweet potato