- Common Markers and Small Molecule Inhibitors in Golgi Studies.
Common Markers and Small Molecule Inhibitors in Golgi Studies.
Methods in molecular biology (Clifton, N.J.) (2022-12-14)
Sarah Bui, Drew Stark, Jie Li, Jianchao Zhang, Yanzhuang Wang
PMID36512231
ABSTRACT
In this chapter, we provide a detailed guide for the application of commonly used small molecules to study Golgi structure and function in vitro. Furthermore, we have curated a concise, validated list of endomembrane markers typically used in downstream assays to examine the consequent effect on the Golgi via microscopy and western blot after drug treatment. This chapter will be useful for researchers beginning their foray into the field of intracellular trafficking and Golgi biology.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Anti-α1,2-Mannosidase IA antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
MG-132, ≥95% by HPLC, Potent, reversible, and cell-permeable proteasome inhibitor (Ki = 4 nM).
Sigma-Aldrich
Bisindolylmaleimide I, A highly selective, cell-permeable, and reversible protein kinase C (PKC) inhibitor (IC₅₀ = 10 nM) that is structurally similar to staurosporine.
Sigma-Aldrich
β-Secretase Inhibitor II, The β-Secretase Inhibitor II, also referenced under CAS 263563-09-3, controls the biological activity of β-Secretase. This small molecule/inhibitor is primarily used for Neuroscience applications.
Millipore
Thapsigargin - CAS 67526-95-8 - Calbiochem, Thapsigargin, CAS 67526-95-8, is a cell-permeable, tumor-promoting sesquiterpene lactone that releases calcium by non-competitvley inhibiting endoplasmic reticular Ca2+-ATPase (IC₅₀ = 4-13 nM).
Sigma-Aldrich
Staurosporine, Streptomyces sp., Staurosporine, CAS 62996-74-, is a cell-permeable, potent, reversible, ATP-competitive inhibitor of protein kinases (IC₅₀ = 7, 20, 1.3, 0.7, & 8.5 nM for PKA, CAMK, MLCK, PKC, & PKG, respectively).
Sigma-Aldrich
Fucosyltransferase Inhibitor, 2F-Peracetyl-Fucose, 2F-Peracetyl-Fucose is a cell-permeable fluorinated fucose derivative that acts as an inhibitor of fucosyltransferases following its uptake and metabolic transformation into a GDP-fucose mimetic.
Sigma-Aldrich
FIBRONECTIN FROM HUMAN PLASMA, liquid, 0.1% (Solution), BioReagent, suitable for cell culture