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  • Quantification of Triphenyl-2H-tetrazoliumchloride Reduction Activity in Bacterial Cells.

Quantification of Triphenyl-2H-tetrazoliumchloride Reduction Activity in Bacterial Cells.

Bio-protocol (2017-01-20)
Roberto Defez, Anna Andreozzi, Carmen Bianco
ABSTRACT

This protocol describes the use of the 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt to evaluate the cell redox potential of rhizobia cells. The production of brightly colored and insoluble 1,3,5-Triphenyltetrazolium formazan arising from TTC reduction is irreversible and can be easily quantified using a spectrophotometer. This protocol allows the production of reproducible results in a relatively short time for Sinorhizobium meliloti 1021 cells grown both in exponential and stationary phases. The results here presented show that the S. meliloti cells deriving from exponential-phase cultures had increased cell redox potential as compared to the ones deriving from stationary-phase cultures. This means that under exponential growth conditions the S. meliloti cells generate higher amount of reducing equivalents needed for TTC reduction.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2,3,5-Triphenyltetrazolium chloride, ≥98.0% (HPLC)
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500 μg
Estimated to ship on May 12, 2025
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CN¥4,055.45
Sigma-Aldrich
Calcium chloride dihydrate, for molecular biology, ≥99.0%
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500 μg
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CN¥4,055.45
Sigma-Aldrich
Dimethyl sulfoxide, ReagentPlus®, ≥99.5%
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SKUPack SizeAvailabilityPriceQuantity
500 μg
Estimated to ship on May 12, 2025
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CN¥4,055.45