- Ataxia telangiectasia derived iPS cells show preserved x-ray sensitivity and decreased chromosomal instability.
Ataxia telangiectasia derived iPS cells show preserved x-ray sensitivity and decreased chromosomal instability.
Scientific reports (2014-06-28)
Yoshihiro Fukawatase, Masashi Toyoda, Kohji Okamura, Ken-ichi Nakamura, Kazuhiko Nakabayashi, Shuji Takada, Mayu Yamazaki-Inoue, Akira Masuda, Michiyo Nasu, Kenichiro Hata, Kazunori Hanaoka, Akon Higuchi, Kaiyo Takubo, Akihiro Umezawa
PMID24970375
ABSTRACT
Ataxia telangiectasia is a neurodegenerative inherited disease with chromosomal instability and hypersensitivity to ionizing radiation. iPS cells lacking ATM (AT-iPS cells) exhibited hypersensitivity to X-ray irradiation, one of the characteristics of the disease. While parental ataxia telangiectasia cells exhibited significant chromosomal abnormalities, AT-iPS cells did not show any chromosomal instability in vitro for at least 80 passages (560 days). Whole exome analysis also showed a comparable nucleotide substitution rate in AT-iPS cells. Taken together, these data show that ATM is involved in protection from irradiation-induced cell death.
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ApopTag ISOL Dual Fluorescence Apoptosis Detection Kit (DNase Types I & II), The ApopTag ISOL Dual Fluorescence Kit utilizes a proprietary double hairpin, dual fluorescently labeled oligonucleotide labeling process to detect & distinguish between typical apoptotic DNA breaks induced by either DNase I or DNase II.