Improving β-glucosidase and xylanase production in a combination of waste substrate from domestic wastewater treatment system and agriculture residues.

Cellulase and hemicellulase activities are considered to the major bottlenecks in the lignocellulosic biorefinery process, especially in an enzyme cocktail lacking β-glucosidase (BGL) and xylanase (XYL). In view of this issue, higher levels of BGL and XYL activities were obtained in the presence of wastewater and activated sludge as an induction medium mixed with 5% of rice straw by Hypocrea sp. W63. The analysis of the ionic content showed that a relatively low sludge dose could enhance the production of BGL and XYL. Most importantly, compared to a medium using freshwater, the proportion of 1:10 sludge to wastewater, which contained nutrient elements, led to 3.4-fold BGL and 3.7-fold XYL production improvements. This research describes the reuse of substrates that are largely and continuously generated from domestic wastewater treatment systems and agriculture residues, which consequently leads to the development of a simultaneous enzyme production process for sustainable biorefinery practices.