Data on gene and protein expression changes induced by apabetalone (RVX-208) in ex vivo treated human whole blood and primary hepatocytes.

Data on gene and protein expression changes induced by apabetalone (RVX-208) in ex vivo treated human whole blood and primary hepatocytes.

Data in brief (2016-08-30)

Sylwia Wasiak, Dean Gilham, Laura M Tsujikawa, Christopher Halliday, Karen Norek, Reena G Patel, Kevin G McLure, Peter R Young, Allan Gordon, Ewelina Kulikowski, Jan Johansson, Michael Sweeney, Norman C Wong

PMID27570805

ABSTRACT

Apabetalone (RVX-208) inhibits the interaction between epigenetic regulators known as bromodomain and extraterminal (BET) proteins and acetyl-lysine marks on histone tails. Data presented here supports the manuscript published in Atherosclerosis "RVX-208, a BET-inhibitor for Treating Atherosclerotic Cardiovascular Disease, Raises ApoA-I/HDL and Represses Pathways that Contribute to Cardiovascular Disease" (Gilham et al., 2016) [1]. It shows that RVX-208 and a comparator BET inhibitor (BETi) JQ1 increase mRNA expression and production of apolipoprotein A-I (ApoA-I), the main protein component of high density lipoproteins, in primary human and African green monkey hepatocytes. In addition, reported here are gene expression changes from a microarray-based analysis of human whole blood and of primary human hepatocytes treated with RVX-208.

MATERIALS

Product Number

Brand

Product Description

401353

Sigma-Aldrich

Goat Anti-Rabbit IgG, H & L Chain Specific Peroxidase Conjugate, liquid, Calbiochem^®

178422

Sigma-Aldrich

Anti-Apolipoprotein A-I Rabbit pAb, liquid, Calbiochem^®

401253

Sigma-Aldrich

Goat Anti-Mouse IgG, H&L Chain Specific Peroxidase Conjugate, liquid, Calbiochem^®

178452

Sigma-Aldrich

Apolipoprotein A-I, Human Plasma, High-Density Lipoprotein, Native apolipoprotein A-I from human plasma. Functions as a cofactor for lecithin-cholesterol acyltransferase. A component of high density lipoprotein.